Majalah Kedokteran Bandung
Polimorfisme Gen MMP-9, Ekspresi MMP-9, dan Indeks Apoptosis Sel Serviks pada Kehamilan 21–36 Minggu PDF Print E-mail
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Wednesday, 25 January 2012 02:08
Udin Sabarudin, Johanes C. Mose, Sofie R. Krisnadi
Departemen Obstetri & Ginekologi, Fakultas Kedokteran Universitas Padjadjaran
Rumah Sakit Dr. Hasan Sadikin Bandung


Abstrak 

Ekspresi berlebihan serta aktivasi beberapa matriks metaloproteinase (MMP) sebelum persalinan mengarah pada degradasi jaringan amnion korion yang secara klinis disebut ketuban pecah dini (KPD). Peningkatan aktivitas MMP akibat polimorfisme gen matrik metaloproteinase-9 (MMP-9) (C-1562T) akan diikuti oleh apoptosis. Penelitian ini bertujuan mencari perbedaan ekspresi MMP-9, indeks apoptosis (IA) sel serviks, serta polimorfisme gen MMP-9 (C-1562T) pada kehamilan 21–36 minggu tanpa dan disertai KPD. Penelitian ini merupakan penelitian kasus kontrol yang dilakukan di RS Dr. Hasan Sadikin dan RS jejaring Bandung (Mei−November 2009). Tidak terdapat hubungan bermakna antara ekspresi MMP-9 dan IA sel serviks dengan setiap variabel pada kedua kelompok. Tiga kasus KPD ditemukan pada ibu berusia di bawah 20 tahun. Usia kehamilan 28−34 minggu memiliki peluang mengalami KPD lebih besar dibandingkan dengan usia kehamilan 21−28 minggu. Ibu hamil dengan indeks massa tubuh (IMT) 19–26 memiliki risiko mengalami KPD. Hanya terdapat satu sampel yang menunjukkan polimorfisme MMP-9 (C-1562T) di kelompok kehamilan 21–36 minggu disertai KPD. Dapat disimpulkan, tidak ditemukan hubungan bermakna antara ekspresi MMP-9 dan IA sel serviks pada kedua kelompok penelitian, begitu pula dengan polimorfisme MMP-9 (C-1562T) yang dapat menyebabkan peningkatan ekspresi MMP-9. [MKB. 2011;43(4):199–206].

Kata kunci: Apoptosis sel serviks, ekspresi MMP-9, ketuban pecah dini, polimorfisme gen MMP-9


MMP-9 Gene Polymorphism, MMP-9 Expression and Cervical Apoptotic Index on 21–36 Weeks of Pregnancy


Abstract

Over expression and premature activation of matrix metalloproteinase (MMP) can lead to degradation of amnion chorionic membrane which clinically called premature rupture of membrane (PROM). Increasing MMP activity caused by matrix metalloproteinase-9 (MMP-9) gene polymorphism (C-1562T) will be followed by apoptosis. This study was aimed to find the differences between MMP-9 expression and cervical apoptotic index (AI) and also MMP-9 (C-1562T) polymorphism on 21–36 weeks of pregnancy with or without PROM. This was case control study and conducted in Dr. Hasan Sadikin Hospital and Bandung Networking Hospitals (May−November 2009). There were no significant correlation between MMP-9 expression and cervical AI in every variable on both groups. Three cases of PROM were found in mothers below 20 years of age. Women with 28−34 weeks of pregnancy had a greater risk for PROM than 21−28 weeks. Pregnant women with body mass index (BMI) 19−26, had risk to have PROM. Only one sample that showed a MMP-9 (C-1562T) polymorphism in the premature labor with PROM group. It can be concluded that there are no significant correlation between MMP-9 expression and cervical cells AI on both groups as well as MMP-9 (C-1562T) polymorphism which can alter MMP-9 expression. [MKB. 2011;43(4):199–206].

Key words: Cervical cell apoptosis, MMP-9 expression, MMP-9 gene polymorphism, premature rupture of membrane 

 
Mutasi Gen NPHS2 (412C→T, 419delG) dan Manifestasi Klinis Sindrom Nefrotik Resisten Steroid Anak Indonesia PDF Print E-mail
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Wednesday, 25 January 2012 02:07
Dedi Rachmadi, Dany Hilmanto, Ponpon Idjradinata, Abdurahman Sukadi
Departemen Ilmu Kesehatan Anak Fakultas Kedokteran Universitas Padjadjaran
Rumah Sakit Dr. Hasan Sadikin Bandung


Abstrak

Sindrom nefrotik resisten steroid (SNRS) karena mutasi gen nephrotic syndrome type 2 (NPHS2) mempunyai manifestasi klinis  lebih berat dibandingkan dengan SNRS tanpa mutasi gen. Penelitian ini bertujuan untuk mengetahui mutasi gen NPHS2 (412C→T, 419delG) pada SNRS anak Indonesia dan melihat perbedaan manifestasi klinisnya. Penelitian ini berupa observasional potong silang pada 88 penderita SNRS yang diambil secara consecutive admission dari 10 rumah sakit pendidikan di Indonesia, periode September 2006–Desember 2007. Analisis mutasi 412C→T dan 419delG diperiksa dengan polymerase chain reaction. Analisis statistik menggunakan multivariat dan uji chi-square. Dari 88 SNRS didapat 58 (66%) mutasi 412C→T dan 69 (78%) mutasi 419delG. Hasil analisis multivariat variabel interval manifestasi klinis (tekanan darah sistol dan diastol, kadar kreatinin serum, usia serangan pertama, dan pertumbuhan) antara kelompok mutasi gen 412C→T, 419delG dan kelompok tanpa mutasi gen didapatkan masing-masing F=0,316; p=0,902 dan F=0,651; p=0,662. Hasil analisis univariat dari variabel nominal manifestasi klinis sebagai berikut: hematuria, p=0,231(0,726); hipertensi, p=0,286 (0,741); usia serangan pertama, p=0,372 (0,304); pertumbuhan skor Z, p=0,087 (0,595), dan kreatinin serum p=0,049 (0,080). Tidak terdapat perbedaan manifestasi klinis antara penderita SNRS dengan mutasi gen NPHS2 (412C→T, 419delG) dan penderita SNRS tanpa mutasi gen, kecuali kadar kreatinin serum pada penderita SNRS dengan mutasi gen 412 C→T. [MKB. 2011;43(4):193–8].

Kata kunci: Manifestasi klinis, mutasi gen NPHS2 (412C→T, 419delG), sindrom nefrotik resisten steroid 
 
                     
NPHS2 (412 C→T and 419delG) Gene Mutation and Their Clinical Manifestation in Indonesian Steroid-Resistant Nephrotic Syndrome 


Abstract

Steroid resistant nephrotic syndrome (SRNS) due to nephrotic syndrome type 2 (NPHS2) gene mutation has more severe clinical manifestation than those without mutation. This study was designed to find NPHS2 gene mutation in 412C→T and 419delG in Indonesian SRNS and to see differences in clinical manifestation. The observational cross sectional study was performed on 88 SRNS. Subjects were taken consecutively from 10 teaching hospitals in Indonesia during September 2006 to December 2007. Analysis for 412 C→T and 419delG mutations were examined by polymerase chain reaction. Multivariate and chi-square-test analysis were used. Of 88 SNRS, 58 (66%) with 412 C→T and 69 (78%) with 419delG mutations. Multivariate analyses for interval variable of clinical manifestations (systolic and diastolic blood pressure, serum creatinin level, age of onset and growth) between SRNS with NPHS2 412C→T, 419delG mutation and SRNS without mutation were F=0.316, p=0.902 and F=0.651, p=0.662, respectively. While univariate analyses for nominal variable of clinical manifestation were as follow: hematuria, p=0.231 (0.726); hypertension, p=0.286 (0.741); age of onset, p=0.372 (0.304); Z-score growth, p=0.087 (0.595) and serum creatinin, p=0.049 (0.08). There is no difference of clinical manifestation between SRNS with NPHS2 412C→T, 419delG mutation and SRNS without mutation, except on serum creatinin in 412C→T mutation. [MKB. 2011;43(4):193–8]. 
 
Key words: Clinical manifestation, NPHS2 (412C→T, 419delG) gene mutation, steroid resistant nephrotic syndrome

 
Paparan Debu Batubara Subkronik pada Peroksidasi Lipid dan Kadar Gula Darah Tikus Diabetes Melitus PDF Print E-mail
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Wednesday, 25 January 2012 02:05
Agus Yuwono,1 Bambang Setiawan,2 Nia Kania,3 Nurdiana,4 M. Aris Widodo4
1Bagian Ilmu Penyakit Dalam, 2Bagian Kimia Kedokteran, 3Bagian Patologi Anatomi RSUD Ulin Banjarmasin, Fakultas Kedokteran Universitas Lambung Mangkurat Banjarbaru
4Bagian Farmakologi, Fakultas Kedokteran Universitas Brawijaya Malang


Abstrak

Di Kalimantan Selatan, prevalensi penderita diabetes melitus sebesar 11,1%. Kalimantan Selatan merupakan provinsi dengan tambang batubara tersebar di seluruh wilayah. Hal ini menjadikan penderita diabetes melitus terpapar debu batubara. Penelitian ini bertujuan untuk mengukur peroksidasi lipid akibat paparan debu batubara subkronik dan pengaruhnya pada kadar gula darah tikus diabetes melitus. Kelompok penelitian meliputi tikus  Wistar diabetes melitus (P1), kelompok diabetes melitus + paparan debu batubara dosis 12,5 mg/m3 1 jam/hari selama 28 hari (P2), dan kelompok diabetes melitus + paparan debu batubara dosis 25 mg/m3 1 jam/hari selama 28 hari (P3), masing-masing 6 ekor. Penelitian dilakukan mulai Agustus–Oktober 2010. Uji analysis of variance (ANOVA) terhadap kadar malondialdehid (MDA) plasma tidak didapatkan peningkatan secara bermakna antara kelompok perlakuan (p>0,05). Penurunan kadar gula untuk P1, P2, dan P3 berturut-turut sebesar 23,6%, 16,9%, dan 9,3%. Analisis uji t tidak berpasangan terhadap kadar gula darah tidak didapatkan perbedaan bermakna sebelum dan setelah perlakuan pada semua kelompok (p>0,05). Tidak terdapat korelasi antara kadar MDA plasma dan kadar gula darah setelah perlakuan pada berbagai kelompok (p>0,05). Disimpulkan bahwa paparan debu batubara subkronik tidak meningkatkan peroksidasi lipid yang mempengaruhi kadar gula darah pada tikus diabetes melitus dan tidak ada korelasi antara kadar MDA dan glukosa darah. [MKB. 2011;43(4):189–92].

Kata kunci: Debu batubara, diabetes melitus, hiperglikemia, peroksidasi lipid


Subchronic Coal Dust Exposure on Lipid Peroxidation and Blood Glucose Level in Diabetes Mellitus Rat


Abstract

In South Kalimantan, prevalence of diabetes mellitus was 11.1%. South Kalimantan is a province with coal mine spread in all districts. This condition induce coal dust exposure on diabetes mellitus patients. Aim of this study was to measure lipid peroxidation by subchronic coal dust exposure and its effect on glucose level in Wistar rats model of diabetes mellitus. Group included diabetes mellitus Wistar rats (P1), diabetes mellitus + coal dust exposure at dose 12.5 mg/m3 1 hour/day for 28 days (P2) and diabetes mellitus + coal dust exposure at dose 25 mg/m3 1 hour/day for 28 days (P3) on 6 rats, respectively. This research was done from August–October 2010. Analysis of variance (ANOVA) test concluded no significant differently on increased plasma malondialdehyde (MDA) level between all groups (p>0.05). Percentage of blood glucose level decreased 23.6%, 16.9% and 9.3% for P1, P2, P3 group, respectively. Unpaired t test concluded that blood glucose level were not significant differently between pre and post treatment in all groups (p>0.05). There was no correlation between plasma MDA level and blood glucose level in all groups of exposure (p>0.05). In conclusions, that subchronic coal dust exposure doesn’t increase lipid peroxidation and no effect on blood glucose level in diabetes mellitus rats and no correlation between MDA dan blood glucose level. [MKB. 2011;43(4):189–92].

Key words: Coal dust, diabetes mellitus, hyperglycemia, lipid peroxidation

 
Susunan Redaksi Majalah Kedokteran Bandung PDF Print E-mail
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Tuesday, 16 December 2008 06:01
 
  PelindungDekan Fakultas Kedokteran Universitas Padjadjaran
  PenasehatPembantu Dekan II
  Penanggung Jawab Dr. Tono Djuwantono, dr., Sp.OG(K)., M.Kes
  Redaksi Senior 
Prof. Herry Garna, dr., Ph.D., Sp.A(K)
  Pemimpin RedaksiDr. Budi Setiabudiawan,dr., Sp.A(K)., M.kes
  Sekretaris Redaksi
Dr. med. Sc. Irma Ruslina Defi, dr., Sp.KFR
  Anggota Redaksi

Dr. Sri Endah Rahayuningsih,dr., Sp.A(K)
Dr. Henni Djuhaeni,dr., MARS
Dini Norviatin, dr.
Marisa Tasya, dr., Sp.OG .
Rd. Reni Ghrahani, dr., Sp.A(K), M.Kes   

  Konsultan Bahasa Inggris

Diba Artsiyanti E. Putri Basar, S.S., M.Si

  SekretariatAti Sulastri, S.Pd
Devi Fabiola Syahfitri, S.Kom
Erlan Aditya Ardiansyah, S.S
Gantini Permanadewi, S.Sos
Ira Andriati A.Md
Rahadian, S.sos 
Last Updated on Tuesday, 07 January 2014 07:50
 
Peran Lem Fibrin Otologus pada Penempelan Tandur Konjungtiva Bulbi Mata Kelinci terhadap Ekspresi Gen Fibronektin dan Integrin PDF Print E-mail
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Wednesday, 25 January 2012 02:03
Sutarya Enus,1 Gantira Natadisastra,1 M. Nurhalim Shahib,2 Rachmat Sulaeman3
1Departemen Ilmu Kesehatan Mata Fakultas Kedokteran Universitas Padjadjaran-Rumah Sakit Cicendo Bandung; 2Departemen BioKimia, 3Departemen Ilmu Penyakit Dalam
Fakultas Kedokteran Universitas Padjadjaran-Rumah Sakit Dr. Hasan Sadikin Bandung


Abstrak

Penempelan jaringan dan penyembuhan luka pada cangkok konjungtiva lebih cepat pada teknik lem fibrin otologus (LFO) dibandingkan dengan teknik jahitan. Kedua proses tersebut memerlukan interaksi fibronektin (FN) dan integrin α5 yang mengaktivasi alur persinyalan intraselular. Tujuan penelitian untuk menentukan kekuatan ekspresi gen FN serta integrin α5 pada kelompok teknik LFO dan jahitan. Uji eksperimental hewan pada kelinci New Zealand White yang terbagi kelompok teknik LFO dan jahitan masing-masing 8 kelinci bertempat di Laboratorium Sentral (Biologi Molekuler) FK Unpad Bandung, periode Mei–Oktober 2008. Sampel jaringan untuk pemeriksaan reverse transcriptase polymerase chain reaction (RT-PCR) diambil dari eksterpasi satu hari sesudah jaringan cangkok konjungtiva bulbi. Analisis data untuk uji hipotesis dengan Mann Whitney for small sample. Ekspresi gen messenger ribonucleic acid (mRNA) FN secara bermakna lebih kuat pada teknik LFO dibandingkan dengan teknik jahitan (1,9 vs 1,0; p=0,014). Tidak terdapat perbedaan bermakna ekspresi gen (mRNA) integrin α5 antara teknik LFO dan teknik jahitan (1,2 vs 1,0; p=0,235). Sebagai simpulan ekspresi gen FN lebih kuat pada teknik LFO dibandingkan dengan jahitan, sedangkan ekspresi gen integrin α5 pada teknik LFO lebih kuat dibandingkan dengan teknik jahitan namun secara statistik tidak bermakna satu hari pascabedah. [MKB. 2011;43(4):183–8].

Kata kunci: Fibronektin, integrin α5, lem fibrin otologus, RT-PCR


The Role of Autologous Fibrin Glue on Attachment Rabbit Conjungtival Graft Based on Fibronectin and Integrin Gene Expression


Abstract

The tissue attachment and wound healing in conjunctional transplantation was more rapid with autologous fibrin glue (AFG) than suture techniques. Both tissue attachment and wound healing process need interaction between fibronectin (FN) dan integrin α5 activating the intra cellular signal transduction pathway. The aim of this study was to evaluate the gene expression, i.e. FN and integrin in conjunctival transplantation, comparing between AFG and suturing techniques. Animal experimental study was done in New Zealand White rabbits, which divided into AFG and suturing technique at Laboratorium Sentral (Biologi Molekuler) FK Unpad Bandung during May–October 2008, each 8 rabbits, respectively. The tissue sample for reverse transcriptase polymerase chain reaction (RT-PCR) examination was taken from the tissue excision one day after conjunctival bulbi transplantation. Data analysis was tested using Mann Whitney for small sample. The FN gene expression power of messenger ribonucleic acid (mRNA) in the AFG technique was stronger than that in suturing technique (1.9 vs 1.0, p=0.014). There was no significant difference in integrin α5 gene expression of mRNA between AFG and suturing techniques (1.2 vs 1.0, p=0.235). In conclusions, FN gene expression in AFG technique is stronger than suturing technique. There is no difference in integrin α5 gene expression between two techniques, however there is a tendency of increased integrin α5 gene expression one day after surgery. [MKB. 2011;43(4):183–8].

Key words: Autologous fibrin glue, fibronectin, integrin α5, RT-PCR

 
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